Measurement

Part:BBa_I20246

Designed by: Jason Kelly, Karen Wong, Nishant Bhat   Group: Endy Lab   (2007-08-15)

A device used to characterize promoters throught measurement of RFP expression

Device is based on I2057 and contains a partial biobrick site (EcoR1, Not1, Spe1) upstream of the RBS. To insert a BB promoter for testing, digest both the promoter and measurement device with EcoR1 and Spe1. Parts can be removed after testing, which facilitates the screening of libraries.

Note: Sequence features are wrong

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 15
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal prefix found in sequence at 1
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 15
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 15
    Illegal AgeI site found at 595
    Illegal AgeI site found at 707
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None